Protocols and Templates

Gene Expression

     Real-time PCR (PDF)
          Tri-reagent Technical Bulletin (from Sigma - (05/08)
          High Capacity cDNA Archive Kit Protocol (from Applied Biosystems - 10/06)
          PerfeCTa SYBR Green SuperMix, ROX Protocol (from Quanta BioSciences - 2007)

     RNA Excel Templates (xlt)
          RNA Dilution Setup (from JVH lab - 06/08)
          High Capacity cDNA Archive Kit Setup (from JVH lab - 06/08)

     Transfection/Reporter Systems (PDF)
          Lipofectamine Reagent Manual (from Invitrogen - 07/04)
          Dual-Luciferase Reporter Assay System Technical Manual (from Promega - 08/06)